季祥1,2，廖利民1，苗小丽1，刘彬1，周志丹1，蔡禄1,2*
(1.内蒙古科技大学生物工程与技术研究所，内蒙古包头 014010；2.内蒙古自治区生物质能源化利用重点实验室，内蒙古包头 014010)
中图分类号：Q946.3 文件标识码：A
摘要：为了提高微藻多糖的提取率，在碱醇提法的基础上，采用单因素和正交试验分别对NaOH浓度、提取温度和提取时间进行了优化。结果表明：该斜生栅藻多糖提取的最佳条件为NaOH浓度0.2mol/L，提取温度80℃，提取时间180 min，在此条件下，斜生栅藻多糖的得率可达到13.94%。优化后斜生栅藻多糖的得率较水提醇沉法和碱醇提法分别提高了92.01%和9.76%。
关键词：微藻；多糖；提取条件优化
Study on extraction process of polysaccharide from the microalgae
JI Xiang1,2, LIAO Limin1,MIAO Xiao-li1, LIU Bin1, Zhou Zhi-dan1, CAI Lu1,2*
(Institute of Bioengineering and Technology, Inner Mongolia University of Science and Technology, Baotou 014010；Inner Mongolia Key Laboratory of Biomass-Energy
Conversion， Baotou, 014010,China)
Abstract: Alkali concentration, extraction temperature and extraction time of the microalgal polysaccharide extraction were optimized by using the mono-factor and the orthogonal experiment to improve the extraction yield of microalgal polysaccharide. The optimum extraction conditions were as follows: the alkali concentration was 0.2mol/L, the extraction temperature was 80℃,and the extraction time was 180 min. Under these conditions, the extraction yield of microalgal polysaccharide was up to 13.94%. Compared with hot water extraction and alkali-alcohol extraction,the extraction yield of microalgal polysaccharide increased by 92.01% and 9.76% ,respectively, after the optimization,。
Keywords: Microalgae; Polysaccharide;Extraction Process Optimization