赵一博，张琦，池俊玲，赵 慧，辛翠花，郭江波
（内蒙古科技大学 生命科学与技术学院，内蒙古 包头 014010）
关键词：砷胁迫；细胞自噬；ATG8f
中图分类号：Q225 文献标识码：A
摘 要：随着国内经济的快速发展，砷污染引发的环境污染问题日益突出。砷可通过食物链最终进入人体，危害人类健康甚至生命安全，因此研究植物响应砷胁迫的分子机制具有现实意义。细胞自噬（autophagy）参与植物响应逆境胁迫，但是尚不清楚其在植物响应砷胁迫过程中的作用。本试验采用不同浓度的As2O3处理野生型拟南芥和过表达型ATG8f的转基因拟南芥，通过对比分析拟南芥植株根长和叶绿素含量的差异，研究过表达细胞自噬基因对植物耐受砷胁迫影响，结果表明过表达ATG8f基因增加了植物对砷胁迫的敏感性。
Impacts on Arsenic Stress Susceptibility in Plants Overexpressing Autophagy-related Gene ATG8f
ZHAO Yi-bo，ZHANG qi，CHI jun-ling， ZHAO Hui，XIN Cui-hua，GUO Jiang-bo
（School of Life Science and Technology , Inner Mongolia University of Science and Technology, Baotou 014010, China）
Key words: Arsenic stress; Autophagy; ATG8f
Abstract: With the rapid economic development, the environmental pollutions caused by arsenic deteriorate has become increasingly acute these years in China. Arsenic is hypertonic and poisonous to people while entering human body via food chain. It is helpful to deeply research the mechanisms of plant responding to arsenic stress. Autophagy involves in response to stresses in plant, but little is known for its roles in plants exposed to arsenic. In order to detect the impact of ATG8f over expression on susceptibility in plant, wildtype Columbia (Col) Arabidopsis and ATG8f transgenic lines were treated by different concentration of arsenic solution. With the comparison of root length and chlorophyll contents, our results showed that the over expression of ATG8f in Arabidopsis increases the susceptibility to arsenic stress.