2.Effects of heavy metals manganese and lead exposure on physiologicaland biochemical indicators in Caenorhabditis elegans

Abstract:

Our study utilized the model organism Caenorhabditis elegans,which shares up to 65% genetic homology with humans,asthe research subject. Following acute exposure,we determined the median lethal concentration,lifespan,locomotor capacity,resistance to oxidative stress,resistance to ultraviolet stress,and the accumulation levels of lipofuscin and reactive oxygen species. This systematic investigation aimed to examine the toxic effects of heavy metals manganese and lead on physiological and biochemical indicators. Results demonstrated that both manganese and lead exposure significantly shortened the nematode’s lifespan (by 40% and 30% ,respectively),accelerating the aging process. Furthermore,manganese and lead exposure markedly inhibited spontaneous movement(Class A movement capacity was reduced by 55% and 30% ,respectively). Concurrently,reactive oxygen species levels were significantly elevated in exposed groups (with fluorescence intensity increasing by 1. 5fold and 1. 2fold under manganese and lead exposure,

Keywords: lead;manganese;caenorhabditis elegans;physiological and biochemical indicators